Biophysical Imaging

Imaging techniques play an important role in many scientific disciplines and especially in biophysical research. In this intensive practical course, students will learn the principles of various imaging techniques based on spectroscopic microscopy [confocal laser scanning microscopy, fluorescence life time imaging and nuclear magnetic resonance imaging] and high-resolution microscopy (atomic force and single-molecule). In addition, principles of image processing, data analysis, and interpretation of multi-dimensional datasets will be treated. The techniques will be illustrated by applications on biological processes, structure-function relationship, assembly and complexation of macro-molecules and the determination of the structure of surfaces.

After successful completion of this course students are expected to be able to:

• explain the basic principles of four spectroscopic methods for imaging of biological or food-related systems:

  • Atomic Force Microscopy (AFM);
  • Laser Scanning Microscopy (LSM);
    -Magnetic Resonance Imaging (MRI);
  • Single-Molecule Localisation Microscopy (SMLM).

• apply these techniques knowing their particular limitations and strengths (achievable spatial resolution, contrast, information content and possible applications);

  • understand and apply suitable image and data processing techniques;
  • interpret the results in terms of (spatial and dynamic) information.

Assumed Knowledge:
Analytical chemistry or spectroscopy and imaging.