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Advanced Optical Imaging & Single-Molecule Biophysics

BIP40306

About this course

In this intensive course, students will learn and apply the working principles and concepts behind various advanced imaging and single-molecule techniques that have revolutionized the way we can monitor inter- and intra-molecular interactions in the life-, materials-, and food sciences. In the first part of the course, the theoretical and experimental background will be taught and practiced during lectures and tutorials. The second part of the course focuses on practical work and applications, offering the student hands-on experience with single-molecule experiments: The students will (1) build and design optical microscopes with the open UC2 microscopy platform, (2) track fluorescently labelled proteins (CRISPR-Cas) in live bacteria, (3) read and distinguish the four DNA bases label-free using nanopore technology, and (4) use FRET as a molecular ruler to accurately determine nanometer distances within single DNA strands. Regarding experimental data analysis, the principles of image processing, interpretation of multi-dimensional datasets, and time trace analysis including machine learning approaches will be treated. All of this prepares the students for the third part of the course, where they will study, discuss, and present some of the latest literature of this dynamic research field. The course is concluded with a written exam.

Learning outcomes

Required prior knowledge

Assumed Knowledge:
BIP24306Spectroscopy and Imaging;
BIP32803 Biophysical Imaging.
Analytical chemistry or similar courses that provide a basic understanding of molecular life sciences / biophysical chemistry topics

Link to more information

If anything remains unclear, please check the FAQ of Wageningen University.

Offering(s)

  • Start date

    13 May 2024

    • Ends
      5 July 2024
    • Term *
      Period 6
    • Location
      Wageningen
    • Instruction language
      English
    Enrolment period closed
  • Start date

    12 May 2025

    • Ends
      4 July 2025
    • Term *
      Period 6
    • Location
      Wageningen
    • Instruction language
      English
    • Register between
      1 Jun, 00:00 - 6 Apr 2025
    Enrolment starts in 2 days
These offerings are valid for students of TU Eindhoven